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Vibriosis of sea-bass (Vibrio anguillarum)

Diagnosis

Mono-Aqua diagnostic kit: It comprises a kit complete with reagents and necessary cards in order to perform a quick (30 sec) sero-agglutination test on bacterial colonies that have been isolated on agar plates from the tissues of sick or suspect fish in the laboratory. Thus, it provides a fast and accurate identification of the pathogen bypassing the need to perform biochemical profiling.

The kit's main novelty lies in the fact that it provides an additional "negative control antiserum", that is, an antiserum that reacts against bacteria with close antigenic properties with Vibrio anguillarum.

When sero-agglutination occurs with the "positive antiserum" it should not happen when the bacterial colonies are tried against the negative control antiserum, providing extra confidence for the result.

A drop of the antiserum is placed on the dark field of the test card. A bacterial colony is taken from the agar plate with the loop and homogenised for 30 sec. with the antiserum. A positive agglutination is characterised by the formation of a multitude of small spherical agglutinates, whereas a negative test shows either no agglutinate formation, or irregularly shaped, cloudy agglutinates.

 

Biomerieux API 20E biochemical profile system:


Most common profiles:
3
047524,
   3047526,    3247524,    3247526

Vibrio anguillarum most often presents the following results on the API 20E microtube test strip:

Abreviation of microtube substrate


Bacterial strain's biochemical attributes


Result


Triad code

OPNG:
ADH:
LDC:

ODC:
CIT:

H
2S:

URE:
TDA:
IND:

VP:
GEL:
GLU:

MAN:
INO:
SOR:

RHA:
SAC:
MEL:

AMY:
ARA:
OX:

β-galactosidase production
Arginine dihydrolase production
Lysine decarboxylase production

Ornithine decarboxylase production
Citrate utilisation
H
2S production

Urease production
Tryptophane deaminase production
Indole production

Acetoin production
Gelatinase production
Acid from glucose

Acid from mannitol
Acid from inositol
Acid from sorbitol

Acid from rhamnose
Acid from sucrose
Acid from melibiose

Acid from amygdalin
Acid from arabinose
Cytochrome oxidase production

+
+
--

--
V
ariable
--

--
--
+

+
+
+

+
--
+

--
+
--

--
V
ariable
+



3



0
or 2



4



7



5



2



4
or 6

 
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Author: Dr. Panos Varvarigos
Freelance Veterinarian - Fish Pathologist, Athens, Greece.


AquaHealthTM
Laboratory.

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Disclaimer:

Every effort has been made to ensure that the information is accurate until the date of last editing. It is based upon the accumulated personal experience of applied veterinary work. The author cannot take responsibility for incorrect interpretation or any resulting consequences. The contents may be used as an educational guide and are definitely not meant to become a stand-alone diagnostic tool or operations manual.

 

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