PRODUCTS AND SERVICES TO FISH
RAPID-DIAGNOSTIC KITS FOR IN VITRO USE
Types used in Greece:
· Mono-aqua -Va detecting Vibrio anguillarum causing Vibriosis
· Mono-aqua -Pp detecting Photobacterium damselae subsp. piscicida causing Pasteurellosis.
· Mono-aqua -Yr detecting Yersinia ruckeri causing Yersiniosis -ERM.
The Mono-Aqua test kits comprise rapid agglutination tests, rapidly verifying (30 sec.) the presence of specific bacteria on cultures. They are destined for laboratory use requiring prior seeding of samples on growth media. They are termed "in vitro" because they are not applied onto live fish (in vivo). The test reagent (specific antiserum) consists of mono-disperse particles coated with antibodies which form a visible granular particle agglutination pattern when mixed with the particular bacterium (antigen) that they are designed to diagnose.
Apart from the test reagent, the kits are delivered with instruction leaflet and analysis cards. In addition, they contain a negative control reagent, consisting also of mono-disperse particles coated with non-specific, but closely related proteins in order to verify the positive result and exclude false positive results for maximum reliability.
Each kit suffices for 50 tests.
The Mono-Aqua rapid agglutination tests are applicable for a rapid preliminary screening of the pathogens isolated on culture, hence speeding bacterial identification and the diagnosis of the most economically important diseases.
The tests are performed by mixing a drop of the test reagent with one or more bacterial colonies, removed from the agar plate, on the dark background of an analysis card. A positive result (specific agglutination) is read as visible white granular particles (agglutinates) of equal size formed within 30sec. on the black card and homogeneously spread over the test zone of the card. (Non-specific, spontaneous agglutination differs from ordinary agglutination by giving non homogeneous flakes as sticky agglutinates on a milky background. Such a result should be considered as negative.)
Verification of the positive result: In case of a positive result with the test reagent then similar application of the negative control reagent on a couple of bacterial colonies from the agar plate verifies against an occasional non-specific agglutination, which, albeit unlikely, may not be ruled out.
When a drop of the negative control reagent is mixed for 30sec with the bacterial colonies on the analysis card, it should not produce an agglutination result similar to the specific agglutination.
The vials with the “test” and “control” reagents.
The Mono-Aqua rapid diagnostic kits are ideal for the fast and safe identification of specific bacteria as well as for the verification of their presence when grown on agar plates mixed with colonies of other bacteria.
The Mono-aqua test kits should be stored refrigerated at +2o to +7o C. Avoid freezing. In a common refrigerator they last for at least 36 months and may be used several times according to needs. Always close vials properly after use.
Copyright © Dr Panos Varvarigos
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